Alteration of Metabolic Conditions Impacts the Regulation of IGF-II/H19 Imprinting Status in Prostate Cancer

Prostate cancer is the second major cause of male deaths. Obesity, type 2 diabetes, and risk are linked. Insulin-like growth factor II (IGF-II) involved in numerous cellular events, including proliferation survival. The IGF-II gene shares its locus with lncRNA, H19. IGF-II/H19 was first to be identified as being “imprinted”—where paternal copy not transcribed—a silencing phenomenon lost many types. We disrupted imprinting behaviour vitro by altering metabolic conditions quantified it using RFLP, qPCR pyrosequencing; changes peptide were measured RIA. tissue samples analysed ddPCR, pyrosequencing IHC. compared silico data, provided TGCA on cBIO Portal. observed disruption behaviour, vitro, a significant increase reciprocal decrease H19 mRNA; increased mRNA translated into peptides. In vivo, most specimens retained status apart from small subset which showed reduced imprinting. A positive correlation seen between expression, concurred findings larger Cancer Genome Atlas (TGCA) cohorts. This did affect peptide. Our show that diabetes and/or obesity, can directly regulation factors carcinogenesis, indirectly suggesting modification lifestyle habits may reduce risk.